CellDETACH™ Temperature-Responsive Cell Culture Surface

* National invention patent (Patent Number: ZL201510780506.3)
* Induces cell shedding simply by lowering the temperature - straightforward, fast, and easy to operate
* No trypsin: preserves cell surface proteins and markers intact
* No cell scraping: avoids mechanical damage to cells and ensures high cell viability
* Optimized cell culture and tissue engineering techniques

Scope of Application
The thermosensitive cell culture surface is suitable for the in vitro culture of most adherent cells, including stem cells, neural cells, macrophages, and cancer cells. It is ideal for harmless cell harvesting, and can be widely used in expanded cell culture, cell therapy, 3D tissue modeling, extracellular matrix research and other fields.
Instructions for Use

In Vitro Cell Culture
1. When the temperature is above 32℃, the thermosensitive coating on the surface of the CellDETACH™ products is in a curled polymer state, showing slight hydrophobicity. This facilitates cell adherence and growth.
2. When the temperature goes below 32°C, the thermosensitive coating on the surface is in a stretched polymer state, which will bind water molecules and expand, showing hydrophilicity. This facilitates the shedding of adherent cells. The shedding efficiency will be at its best when the temperature drops below 4 °C.
3.When the temperature of the thermosensitive cell culture surface drops below 32°C, excessive disturbance may cause cells to fall off, so please do not spend too much time taking pictures and observing while performing the cell culture.

Cell Harvest
* The best harvest is achieved when the confluence of cells is higher than 80%.
* When harvesting cells, either the environmental temperature should be reduced to 4 ℃ , or the thermosensitive products should be placed in a sterile incubator at  4 ℃, or the culture can be replaced with a 4 °C culture medium.
* When the temperature of the thermosensitive Cell Culture Surface drops to 4 ℃, keep it for 20 ~ 30 minutes, then aspirate the culture medium above the thermosensitive Cell Culture Surface with a straw (cell culture dish), pipette or electric pipette (600ml Cell Culture Flask), and blow over the cells attached to the culture surface to make them fall off. During the blowing process, the cell sheet can be observed falling off the thermosensitive surface.
* The thermosensitive shedding abilities of cells depends on their adhesion performances, and some cells with strong adherence may have difficulty falling off, requiring multiple blowing attempts. (for example, those digested at 37 ℃ for more than 3 minutes with 0.25% trypsin digestion are considered to be cells with strong adherence ability).
Storage and Transportation
* This product should not be exposed to direct sunlight or excessive heat for a long time, but can be stored and transported at room temperature.